Flag-Tag Monoclonal Antibody

Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. 

Research Area: Tags and Cell Markers

Synonyms:
DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag

Immunogen: Synthetic Peptide

Purification Method: Protein A purification

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Concentration: 1 mg/mL
Dilution: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300

Storage:
Store at -20°C. Avoid freeze / thaw cycles.