FLT3 Ligand human active recombinant protein
€295.00
In stock
SKU
600114
Protein Family: Cytokines
Pathway and Disease: Immune System, Signaling Molecules and Interaction, Cancers, Cell Growth and Death, Signal Transduction
Alternate Names: SL cytokine, Fms-related tyrosine kinase receptor 3 ligand, FLT3L, Flt3 ligand, FLT3LGDescription:
Fms-related tyrosine kinase receptor 3 ligand (Flt3 ligand, FLT3LG) binds to Fms-Like Tyrosine Kinase-3 (FLT-3). Human FLT3 ligand stimulates the proliferation of cells expressing murine FLT-3 receptors. The effects of FLT-3 ligand are synergized by coexpression of G-CSF, GM-CSF, M-CSF, IL3 and SCF. FLT-3 ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3. Recombinant Human Flt3-Ligand produced in E. coli is a non-glycosylated polypeptide chain containing 155 amino acids with a MW of 17,605 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
rHuFlt3-Ligand is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is expected in a range of 0.6 - 1.0 ng/ml.
Accession No.: P49771
MW: 17605 Da
Sequence:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signaling Molecules and Interaction, Cancers, Cell Growth and Death, Signal Transduction
Alternate Names: SL cytokine, Fms-related tyrosine kinase receptor 3 ligand, FLT3L, Flt3 ligand, FLT3LGDescription:
Fms-related tyrosine kinase receptor 3 ligand (Flt3 ligand, FLT3LG) binds to Fms-Like Tyrosine Kinase-3 (FLT-3). Human FLT3 ligand stimulates the proliferation of cells expressing murine FLT-3 receptors. The effects of FLT-3 ligand are synergized by coexpression of G-CSF, GM-CSF, M-CSF, IL3 and SCF. FLT-3 ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3. Recombinant Human Flt3-Ligand produced in E. coli is a non-glycosylated polypeptide chain containing 155 amino acids with a MW of 17,605 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
rHuFlt3-Ligand is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is expected in a range of 0.6 - 1.0 ng/ml.
Accession No.: P49771
MW: 17605 Da
Sequence:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
| Is Featured? | No |
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