Free Cholesterol (FC) Colorimetric Assay Kit

Detection Principle:
Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Instrument: Microplate reader
Assay Time: 20 min

Tested Sample Types: Serum, plasma, tissue

Research Area's: Lipids metabolism

Shelf Life: 6 Months

Storage:
2-8℃