Fungiplex® Candida IVD PCR

Fungiplex® Candida IVD PCR
Bruker’s Fungiplex® Candida IVD PCR Kit is a multiplex real-time PCR test for the most common pathogens associated with Invasive Candidiasis (IC); providing rapid results with high sensitivity and specificity without blood culture. In most cases, clinicians do not have time to wait for culture, especially in patients with sepsis. The Fungiplex Candida assay enables the early identification of Candida infection in a culture-free workflow.

Invasive Candidiasis
IC is difficult to diagnose and is associated with high morbidity and mortality. Blood cultures exhibit a sensitivity of only 50% for diagnosing IC, and become positive late in the disease. Fungiplex Candida enables the rapid identification of Candida species from blood samples, without the need to wait for blood culture.

Clinical problem
IC affects immunocompromised and critical care patients. Conventional diagnostic techniques are not compatible with the timely diagnosis of IC, and exhibit poor sensitivity. Delays in initiating antifungal therapy in patients with candidaemia dramatically increase mortality rates.
- Blood cultures exhibit poor sensitivity for IC and become positive late in the disease
- Delayed diagnosis of IC is associated with increased mortality and prolonged hospital stays
- Empiric treatment is often employed, which results in overuse of antifungal agents and exacerbates the growing threat of multi-drug resistance.

Molecular testing for Candida
Fungiplex Candida is a multiplex real-time PCR assay, which rapidly detects the main causative species associated with IC, and differentiates those resistant to first-line treatment:
- Candida spp. (C. albicans, C. parapsilosis, C. dubliniensis, C. tropicalis)
- Candida glabrata
- Candida krusei

Rapid results
Fungiplex Candida is a direct testing assay, providing results in under 2 hours from extracted DNA, allowing rapid reporting from the laboratory to support clinical decision-making when time matters. It is validated for use on DNA extracted from whole blood, serum and plasma without the need for culture.

Compatibility
Fungiplex Candida is provided in an easy to use master-mix format and is designed to be compatible with existing laboratory equipment, minimizing the need for dedicated bench space or additional training:
- ABI 7500br />- ABI QuantStudio 5
- Bio-Rad CFX
- Hain FluoroCycler® XT
- Mic qPCR Cycler
- Roche Lightcycler 480 II
- QIAGEN RotorGene.
Fungiplex Candida is designed to run on the same thermocycler conditions as Fungiplex Aspergillus, allowing clinics and laboratories to conduct testing with flexibility and efficiency.

More information
Fungiplex® Candida IVD PCR (Brochure)
Fungiplex® Candida Instructions for Use