G-CSF human active recombinant protein
€295.00
In stock
SKU
600118
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Granulocyte colony-stimulating factor, G-CSF, pluripoietin, filgrastim, lenograstim, CSF3, GCSFDescription:
Human Granulocyte colony-stimulating factor (G-CSF) induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. Secretion of G-CSF in epithelial, endothelial and fibroblastic cells is induced by Interleukin-17. Recombinant Human G-CSF produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids with a MW of 18.8 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Human Granulocyte-Colony Stimulating Factor is fully biologically active when compared to standard. The ED50 was calculated by the dose-dependent proliferation of mouse NFS-60 is less then 0.1 ng/ml.
Accession No.: P09919
MW: 18800 Da
Sequence:
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium citrate, pH 3.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Granulocyte colony-stimulating factor, G-CSF, pluripoietin, filgrastim, lenograstim, CSF3, GCSFDescription:
Human Granulocyte colony-stimulating factor (G-CSF) induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. Secretion of G-CSF in epithelial, endothelial and fibroblastic cells is induced by Interleukin-17. Recombinant Human G-CSF produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids with a MW of 18.8 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Human Granulocyte-Colony Stimulating Factor is fully biologically active when compared to standard. The ED50 was calculated by the dose-dependent proliferation of mouse NFS-60 is less then 0.1 ng/ml.
Accession No.: P09919
MW: 18800 Da
Sequence:
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium citrate, pH 3.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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