G-CSF mouse active recombinant protein
€295.00
In stock
SKU
600184
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Granulocyte colony-stimulating factor, G-CSF, CSF3, GCSF, CsfgDescription:
Granulocyte colony-stimulating factor (G-CSF) induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. Secretion of G-CSF in epithelial, endothelial, and fibroblastic cells is induced by Interleukin-17. Recombinant Mouse G-CSF produced in E. coli is a single non-glycosylated polypeptide chain containing 179 amino acids with a MW of 19 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Mouse recombinant G-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of mouse M-NFS-60 cells, is 0.04 ng/ml.
Accession No.: P09920
MW: 19000 Da
Sequence:
MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Granulocyte colony-stimulating factor, G-CSF, CSF3, GCSF, CsfgDescription:
Granulocyte colony-stimulating factor (G-CSF) induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. Secretion of G-CSF in epithelial, endothelial, and fibroblastic cells is induced by Interleukin-17. Recombinant Mouse G-CSF produced in E. coli is a single non-glycosylated polypeptide chain containing 179 amino acids with a MW of 19 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Mouse recombinant G-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of mouse M-NFS-60 cells, is 0.04 ng/ml.
Accession No.: P09920
MW: 19000 Da
Sequence:
MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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