Galactose Separopore® 4B-CL (Divinyl Sulfone-Coupled)
€0.00
In stock
SKU
BW-20181173
Application:
Galactose coupled Separopore® is a high strength bioagarose matrix.
Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.
This purification method is milder and/or more specific than other methods, allowing protein recovery in a manner that preserves activity for downstream Application: .
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: D-Galactose Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 - 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Chemical linkage: Divinyl Sulfone
Spacer: 5 atom
Binding capacity: >20 mg jacalin / ml drained gel
pH stability: 4 - 9
Flow Specifications: 70 - 140 cm / h
Supplied as a slurry in 20% ethanol
References:
Affinity purification of recombinant cholera toxin B subunit oligomer expressed in Bacillus brevis for potential human use as a mucosal adjuvant. FEMS Immunol Med Microbiol. (1998) 20: 311-8.
Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
A crude extract of Artocarpus integrifolia contains two lectins with distinct biological activities. Immunol Lett. (1992) 31: 65-71.
Two asialoglycoprotein receptor polypeptides in human hepatoma cells. J Biol Chem. (1987) 262: 11825-32.
Identification of rat testis galactosyl receptor using antibodies to liver asialoglycoprotein receptor: purification and localization on surfaces of spermatogenic cells and sperm. J Cell Biol. (1989) 108: 367-75.
A D-galactose-binding lectin with mitogenic activity from Musca domestica pupae. Zoolog Sci. (2009) 26: 249-53.
Autoantibodies to asialoglycoprotein receptor (ASGPR) measured by a novel ELISA--revival of a disease-activity marker in autoimmune hepatitis. Clin Chim Acta. (2009) 408: 19-24.
The unique enzymatic function of field bean (Dolichos lablab) D-galactose specific lectin: a polyphenol oxidase. Glycoconj J. (2009) 26: 535-45.
The amino acids involved in the distinct carbohydrate specificities between macrophage galactose-type C-type lectins 1 and 2 (CD301a and b) of mice. Biochim Biophys Acta. (2008) 1780: 89-100.
Balamuthia mandrillaris interactions with human brain microvascular endothelial cells in vitro. J Med Microbiol. (2007) 56: 1110-5.
Differential structure and activity between human and mouse intelectin-1: human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomer. Glycobiology. (2007) 17: 1045-51.
Isolation, characterization, and extra-embryonic secretion of the Xenopus laevis embryonic epidermal lectin, XEEL. Glycobiology. (2005) 15: 281-90.
One- and two-dimensional gel electrophoresic identification of African yam bean seed proteins. J Agric Food Chem. (2000) 48: 2296-9.
Isolation and partial characterisation of galactose-specific lectins from African yam beans, Sphenostyles stenocarpa Harms. Phytochemistry. (1999) 51: 721-8.
Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen. J Immunol. (1996) 156: 128-35.
Surfactant protein A amino acids Glu195 and Arg197 are essential for receptor binding, phospholipid aggregation, regulation of secretion, and the facilitated uptake of phospholipid by type II cells. J Biol Chem. (1994) 269: 29801-7.
Purification and characterization of Silurus asotus (catfish) roe lectin. Biol Pharm Bull. (1993) 16: 1-5.
Cloning, sequence analysis and expression in Escherichia coli of the cDNA encoding a precursor of peanut agglutinin. FEBS Lett. (1992) 307: 185-9.
Activation of T and B cells by a crude extract of Artocarpus integrifolia is mediated by a lectin distinct from jacalin. J Immunol Methods. (1991) 140: 197-203.
Carbohydrate-recognition domains as tools for rapid purification of recombinant eukaryotic proteins. Biochem J. (1991) 274: 575-80.
Purification and characterization of a new type lactose-binding Ulex europaeus lectin by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 95-102.
Purification and characterization of two types of Cytisus sessilifolius anti-H(O) lectins by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 103-11.
Comparative studies of carbohydrate-binding proteins from Xenopus laevis skin and eggs. Sugar-binding specificities and affinity purification. Chem Pharm Bull (Tokyo). (1990) 38: 975-81.
The rat liver asialoglycoprotein receptor polypeptide must be inserted into a microsome to achieve its active conformation. J Biol Chem. (1986) 261: 4940-7.
Cytotonic enterotoxins and cytotoxic factors produced by Salmonella enteritidis and Salmonella typhimurium. Toxicon. (1983) 21: 785-96.
Related products:
Dextran-Separopore® 4B-CL (SKU # 20181005)
Fucose-Separopore® 4B-CL (SKU # 20181010)
Glucose-Separopore® 4B-CL (SKU # 20181076)
Lactose-Separopore® 4B-CL (SKU # 20181017)
Maltose-Separopore® 4B (SKU # 20181072)
Mannose-Separopore® 4B-CL (SKU # 20181019)
Guar Gum-Separopore® 4B-CL (SKU # 20181052)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-Hazardous
Storage: 2-8°C
Appearance Form: Liquid (Suspension)
Brand Name: bioPLUS™, Separopore®
Galactose coupled Separopore® is a high strength bioagarose matrix.
Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.
This purification method is milder and/or more specific than other methods, allowing protein recovery in a manner that preserves activity for downstream Application: .
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: D-Galactose Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 - 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Chemical linkage: Divinyl Sulfone
Spacer: 5 atom
Binding capacity: >20 mg jacalin / ml drained gel
pH stability: 4 - 9
Flow Specifications: 70 - 140 cm / h
Supplied as a slurry in 20% ethanol
References:
Affinity purification of recombinant cholera toxin B subunit oligomer expressed in Bacillus brevis for potential human use as a mucosal adjuvant. FEMS Immunol Med Microbiol. (1998) 20: 311-8.
Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
A crude extract of Artocarpus integrifolia contains two lectins with distinct biological activities. Immunol Lett. (1992) 31: 65-71.
Two asialoglycoprotein receptor polypeptides in human hepatoma cells. J Biol Chem. (1987) 262: 11825-32.
Identification of rat testis galactosyl receptor using antibodies to liver asialoglycoprotein receptor: purification and localization on surfaces of spermatogenic cells and sperm. J Cell Biol. (1989) 108: 367-75.
A D-galactose-binding lectin with mitogenic activity from Musca domestica pupae. Zoolog Sci. (2009) 26: 249-53.
Autoantibodies to asialoglycoprotein receptor (ASGPR) measured by a novel ELISA--revival of a disease-activity marker in autoimmune hepatitis. Clin Chim Acta. (2009) 408: 19-24.
The unique enzymatic function of field bean (Dolichos lablab) D-galactose specific lectin: a polyphenol oxidase. Glycoconj J. (2009) 26: 535-45.
The amino acids involved in the distinct carbohydrate specificities between macrophage galactose-type C-type lectins 1 and 2 (CD301a and b) of mice. Biochim Biophys Acta. (2008) 1780: 89-100.
Balamuthia mandrillaris interactions with human brain microvascular endothelial cells in vitro. J Med Microbiol. (2007) 56: 1110-5.
Differential structure and activity between human and mouse intelectin-1: human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomer. Glycobiology. (2007) 17: 1045-51.
Isolation, characterization, and extra-embryonic secretion of the Xenopus laevis embryonic epidermal lectin, XEEL. Glycobiology. (2005) 15: 281-90.
One- and two-dimensional gel electrophoresic identification of African yam bean seed proteins. J Agric Food Chem. (2000) 48: 2296-9.
Isolation and partial characterisation of galactose-specific lectins from African yam beans, Sphenostyles stenocarpa Harms. Phytochemistry. (1999) 51: 721-8.
Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen. J Immunol. (1996) 156: 128-35.
Surfactant protein A amino acids Glu195 and Arg197 are essential for receptor binding, phospholipid aggregation, regulation of secretion, and the facilitated uptake of phospholipid by type II cells. J Biol Chem. (1994) 269: 29801-7.
Purification and characterization of Silurus asotus (catfish) roe lectin. Biol Pharm Bull. (1993) 16: 1-5.
Cloning, sequence analysis and expression in Escherichia coli of the cDNA encoding a precursor of peanut agglutinin. FEBS Lett. (1992) 307: 185-9.
Activation of T and B cells by a crude extract of Artocarpus integrifolia is mediated by a lectin distinct from jacalin. J Immunol Methods. (1991) 140: 197-203.
Carbohydrate-recognition domains as tools for rapid purification of recombinant eukaryotic proteins. Biochem J. (1991) 274: 575-80.
Purification and characterization of a new type lactose-binding Ulex europaeus lectin by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 95-102.
Purification and characterization of two types of Cytisus sessilifolius anti-H(O) lectins by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 103-11.
Comparative studies of carbohydrate-binding proteins from Xenopus laevis skin and eggs. Sugar-binding specificities and affinity purification. Chem Pharm Bull (Tokyo). (1990) 38: 975-81.
The rat liver asialoglycoprotein receptor polypeptide must be inserted into a microsome to achieve its active conformation. J Biol Chem. (1986) 261: 4940-7.
Cytotonic enterotoxins and cytotoxic factors produced by Salmonella enteritidis and Salmonella typhimurium. Toxicon. (1983) 21: 785-96.
Related products:
Dextran-Separopore® 4B-CL (SKU # 20181005)
Fucose-Separopore® 4B-CL (SKU # 20181010)
Glucose-Separopore® 4B-CL (SKU # 20181076)
Lactose-Separopore® 4B-CL (SKU # 20181017)
Maltose-Separopore® 4B (SKU # 20181072)
Mannose-Separopore® 4B-CL (SKU # 20181019)
Guar Gum-Separopore® 4B-CL (SKU # 20181052)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-Hazardous
Storage: 2-8°C
Appearance Form: Liquid (Suspension)
Brand Name: bioPLUS™, Separopore®
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