Gamma-Catenin Phospho-Regulation Antibody Sampler Kit

Background:
Plakoglobin (γ-catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues (Tyr-133, Tyr-480, Tyr-550, Tyr-644) in γ-catenin can modify its interactions with other proteins. Phosphorylation of Tyr-644 decreases γ-catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate Tyr-550, which increases γ-catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-catenin protein-protein interactions within desmosomal complexes.

Buffer/Storage:
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.