GAPDH Monoclonal Antibody
€0.00
In stock
SKU
E-AB-20032
Catalog Number: E-AB-20032
Isotype: Mouse IgG, Clone: 9S5
Reactivity: human, mouse, rat, monkey, cow, dog, hamster, pig, rabbit, sheep, insect, yeast
Applications: WB,IHC-p,IF
Datasheet, Questions? Contact us!
Isotype: Mouse IgG, Clone: 9S5
Reactivity: human, mouse, rat, monkey, cow, dog, hamster, pig, rabbit, sheep, insect, yeast
Applications: WB,IHC-p,IF
Datasheet, Questions? Contact us!
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Research Area: Cancer, Metabolism, Signal Transduction
Synonyms:
Aging associated gene 9 protein, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, Peptidyl-cysteine S-nitrosylase GAPDH
Immunogen: Synthetic Peptide
Swissprot: P04406
Observed MW: 37 kDa
Purification Method: Protein A purification
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
https://www.molbiolcell.org/doi/abs/10.1091/mbc.E18-12-0770
https://pubmed.ncbi.nlm.nih.gov/31783627/
https://pubmed.ncbi.nlm.nih.gov/31024805/
https://pubmed.ncbi.nlm.nih.gov/32821143/
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Research Area: Cancer, Metabolism, Signal Transduction
Synonyms:
Aging associated gene 9 protein, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, Peptidyl-cysteine S-nitrosylase GAPDH
Immunogen: Synthetic Peptide
Swissprot: P04406
Observed MW: 37 kDa
Purification Method: Protein A purification
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
https://www.molbiolcell.org/doi/abs/10.1091/mbc.E18-12-0770
https://pubmed.ncbi.nlm.nih.gov/31783627/
https://pubmed.ncbi.nlm.nih.gov/31024805/
https://pubmed.ncbi.nlm.nih.gov/32821143/
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