GAPDH Polyclonal Antibody

Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Research Area: Cancer, Metabolism, Signal Transduction

Synonyms:
Aging associated gene 9 protein, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, Peptidyl-cysteine S-nitrosylase GAPDH

Immunogen: Synthesized peptide derived from the N-terminal region of human GAPDH.

Swissprot: P04406
Observed MW: 36 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
http://www.sciencedirect.com/science/article/pii/S0753332216317309
https://pubmed.ncbi.nlm.nih.gov/31586451/
https://pubmed.ncbi.nlm.nih.gov/32407635/