General 5-Me-THF (5-Methyltetrahydrofolate) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Methyltetrahydrofolate(5-Me-THF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Methyltetrahydrofolate(5-Me-THF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Methyltetrahydrofolate(5-Me-THF) in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names:
5-Me-THFA, 5-Me-H4FA, 5-Me-THF, 5-Me-H4F, Levomefolic Acid, Metafolin, 5-Methyltetrahydrofolic Acid, L-Methylfolate

Sample Type:
serum, plasma and other biological fluids

Assay Type: Competitive Inhibition

Standard: 90 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only