General Hyp (Hydroxyproline) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hydroxyproline(Hyp) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxyproline(Hyp). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxyproline(Hyp) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Nutrition metabolism,

Alternative Names:
(2S,4R)-4-Hydroxyproline, L-Hydroxyproline(C5H9O3N)

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Competitive Inhibition

Standard: 5000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only