General PEG (Polyethylene Glycol) ELISA Kit

General PEG (Polyethylene Glycol) ELISA Kit

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In stock
SKU
ELK8264
Catalog Number: ELK8264
Reactivity: General
Standard Range: 15.63-1000 ng/mL
Sensitivity: 4.72 ng/mL
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Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Polyethylene Glycol(PEG) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polyethylene Glycol(PEG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polyethylene Glycol(PEG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names:
PEO, POE, Polyethylene oxide, Polyoxyethylene, Carbowax, GoLYTELY, GlycoLax, Fortrans, TriLyte, Colyte, Halflytely, Macrogol, MiraLAX, MoviPrep

Sample Type:
biological agents

Assay Type: Competitive Inhibition

Standard: 1000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only
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