General PP (Dipalmitin) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dipalmitin(PP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipalmitin(PP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipalmitin(PP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names:
1,3-Dipalmitin, 1,3-Di-(Hexadecanoyl)-Glycerol, 1,3-Dipalmitoylglycerol, 1,3-Dipalmitoyl-Glycerol, Glyceryl 1,3-Dipalmitate

Sample Type:
biological agents

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only