General THB (Tetrahydrobiopterin) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin (THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin (THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin (THB) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Genetic diseases

Alternative Names:
Tetrahydrobiopterin, BH4, Kuvan, Sapropterin

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Competitive Inhibition

Standard: 100 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only