General VB6 (Vitamin B6) ELISA Kit

General VB6 (Vitamin B6) ELISA Kit

€0.00
In stock
SKU
ELK7889
Catalog Number: ELK7889
Reactivity: General
Standard Range: 4.69-300 ng/mL
Sensitivity: 1.42 ng/mL
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Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin B6(VB6) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin B6(VB6). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin B6(VB6) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Nutrition metabolism, Hormone metabolism,

Alternative Names:
Pyridoxine

Sample Type:
serum, plasma and other biological fluids

Assay Type: Competitive Inhibition

Standard: 300 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only
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