Glial Fibrillary Acidic Protein (clone GA-5), anti-human, mouse, rat pig, chicken

Source:
A BALB/c mouse was immunized with a crude preparation from porcine spinal cord.

Specifications:
G-A-5 reacts specifically with Glial Fibrillary Acid Protein. GFAP is the major protein of glial filaments in differentiated astrocytes and ependymal cells. It has a MW of 52 kDa and in the peripheral nervous system GFAP has been demonstrated in Schwann cells.

Species reactivity:
Positive: chicken, human, mouse, pig, rat.

Applications:
G-A-5 is particularly useful for identifying GFAP in frozen and formalin-fixed paraffin-embedded brain tissue sections. In neoplastic tissue G-A-5 is positive in astrocytoma’s and ependymomas. G-A-5 can also be applied for Western blot analysis.
Frozen sections: +
Paraffin sections: Citrate
Western blotting: +

Positive control: Brain, neuroblastoma.

References:
- Debus E., et al, Differentiation25(2): 193-203 (1983).
- Kobayashi K. et al, Brain Res.740(1-2): 57-65 (1996)