Glucose Uptake Fluorometric Assay Kit

Detection Principle:
2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Instrument: Fluorescence microplate reader
Assay Time: 100 min

Tested Sample Types: Cells

Research Area's: Others

Shelf Life: 6 Months

Storage:
-20℃