Glutathione Red Separopore® 4B Microbeads viewIP™
€0.00
In stock
SKU
BW-20181116
Application:
Red-colored affinity matrix consists of reduced glutathione covalently attached to red beaded agarose 4B (Separopore®) via epoxy activation. View-IP™ Glutathione Red-Separopore® 4B-CL is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, i.e. glutathione S-transferase (GST), glutathione peroxidase and glyoxalase I. It greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reduced glutathione (GSH)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Ligand coupling method: Epoxy activation
Exclusion limit: approximately 2 x 107
Binding capacity: > 5 mg GST/ml drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 2 – 8 °C
References:
Optimized refolding and characterization of active C-terminal ADAMTS-18 fragment from inclusion bodies of Escherichia coli. Protein Expr Purif. (2012) 82: 32-6.
Purification and biochemical characterization of glutathione S-transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Arch Insect Biochem Physiol. (2011) 78: 201-15.
Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. (2011) 286: 29671-80.
Expression and purification of 15N- and 13C-isotope labeled 40-residue human Alzheimer's β-amyloid peptide for NMR-based structural analysis. Protein Expr Purif. (2011) 79: 16-24.
Detection of anti-oxidant enzymatic activities and purification of glutathione transferases from Angiostrongylus cantonensis. Exp Parasitol. (2011) 127: 365-9.
Preparation of insoluble GST fusion proteins. Cold Spring Harb Protoc. (2009) 2009: pdb.prot4997.
Preparation of soluble GST fusion proteins. Cold Spring Harb Protoc. (2009) 2009: pdb.prot4996.
Purification and partial characterization of glutathione S-transferase from insecticide-resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae). Arch Insect Biochem Physiol. (2009) 70: 136-50.
Ubiquitination as a priming process of PKC alpha and PKC epsilon degradation in the alphaT3-1 gonadotrope cell line. Neuroendocrinology. (2009) 89: 252-66.
A potent reporter applicable to the monitoring of caspase-3-dependent proteolytic cleavage. J Biotechnol. (2008) 138: 17-23.
High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris. Protein Expr Purif. (2008) 60: 1-6.
Expression and purification of glutathione-S-transferase fusion proteins. Curr Protoc Mol Biol. (2001) 16: Unit16.7.
Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes. Protein Pept Lett. (2008) 15: 6-12.
Preparation and properties of recombinant rat and human procholecystokinin(57-95). Protein J. (2008) 27: 186-91.
Purification of CK1 by affinity chromatography on immobilised axin. Protein Expr Purif. (2007) 54: 101-9.
A Mu-class glutathione S-transferase from gills of the marine shrimp Litopenaeus vannamei: purification and characterization. J Biochem Mol Toxicol. (2007) 21: 62-7.
The expression and purification of the N-terminal activation domain of the transcription factor c-Myc: a model substrate for exploring ERK2 docking interactions. Protein Expr Purif. (2007) 53: 80-6.
Recombinant omega-conotoxin MVIIA possesses strong analgesic activity. BioDrugs. (2006) 20: 275-81.
Elevated K-ras activity with cholestyramine and lovastatin, but not konjac mannan or niacin in lung--importance of mouse strain. Biochem Pharmacol. (2006) 72: 1749-55.
A radioassay for synaptic core complex assembly: screening of herbal extracts for effectors. Anal Biochem. (2006) 357: 50-7.
A pull-down assay for 5' AMP-activated protein kinase activity using the GST-fused protein. Mol Biotechnol. (2006) 32: 17-21.
Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beads. Anal Biochem. 2005 Dec 1;347(1):67-76.
Expression and purification of the mitochondrial serine protease LACTB as an N-terminal GST fusion protein in Escherichia coli. Protein Expr Purif. (2006) 45: 335-42.
Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5). Protein Expr Purif. (2006) 45: 352-8.
Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2. J Biol Chem. (2005) 280: 35329-36.
Identification and characterization of a selenium-dependent glutathione peroxidase in Setaria cervi. Biochem Biophys Res Commun. (2005) 331: 1069-74.
Recombinant expression of maize nucleotide excision repair protein Rad23 in Escherichia coli. Protein Expr Purif. (2005) 41: 287-97.
An alternative use of basic pGEX vectors for producing both N- and C-terminal fusion proteins for production and affinity purification of antibodies. Protein Expr Purif. (2005) 40: 287-91.
Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Parasite Immunol. (2004) 26: 351-8.
Interaction of the Hsp90 cochaperone cyclophilin 40 with Hsc70. Cell Stress Chaperones. (2004) 9: 167-81.
Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella. Reprod Biol Endocrinol. (2004) 2: 57.
14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain. J Biol Chem. (2004) 279: 26105-14.
Abiotic stress alters transcript profiles and activity of glutathione S-transferase, glutathione peroxidase, and glutathione reductase in Euphorbia esula. Physiol Plant. (2004) 120: 421-433.
Analysis of specific interactions of native protein phosphatase 1 isoforms with targeting subunits. Methods Enzymol. (2003) 366: 156-75.
The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly. J Biol Chem. (2003) 278: 27644-51.
Preparation of biologically active recombinant human progastrin(1-80). J Protein Chem. (2002) 21: 465-71.
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
Red-colored affinity matrix consists of reduced glutathione covalently attached to red beaded agarose 4B (Separopore®) via epoxy activation. View-IP™ Glutathione Red-Separopore® 4B-CL is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, i.e. glutathione S-transferase (GST), glutathione peroxidase and glyoxalase I. It greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reduced glutathione (GSH)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Ligand coupling method: Epoxy activation
Exclusion limit: approximately 2 x 107
Binding capacity: > 5 mg GST/ml drained gel
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 2 – 8 °C
References:
Optimized refolding and characterization of active C-terminal ADAMTS-18 fragment from inclusion bodies of Escherichia coli. Protein Expr Purif. (2012) 82: 32-6.
Purification and biochemical characterization of glutathione S-transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Arch Insect Biochem Physiol. (2011) 78: 201-15.
Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. (2011) 286: 29671-80.
Expression and purification of 15N- and 13C-isotope labeled 40-residue human Alzheimer's β-amyloid peptide for NMR-based structural analysis. Protein Expr Purif. (2011) 79: 16-24.
Detection of anti-oxidant enzymatic activities and purification of glutathione transferases from Angiostrongylus cantonensis. Exp Parasitol. (2011) 127: 365-9.
Preparation of insoluble GST fusion proteins. Cold Spring Harb Protoc. (2009) 2009: pdb.prot4997.
Preparation of soluble GST fusion proteins. Cold Spring Harb Protoc. (2009) 2009: pdb.prot4996.
Purification and partial characterization of glutathione S-transferase from insecticide-resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae). Arch Insect Biochem Physiol. (2009) 70: 136-50.
Ubiquitination as a priming process of PKC alpha and PKC epsilon degradation in the alphaT3-1 gonadotrope cell line. Neuroendocrinology. (2009) 89: 252-66.
A potent reporter applicable to the monitoring of caspase-3-dependent proteolytic cleavage. J Biotechnol. (2008) 138: 17-23.
High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris. Protein Expr Purif. (2008) 60: 1-6.
Expression and purification of glutathione-S-transferase fusion proteins. Curr Protoc Mol Biol. (2001) 16: Unit16.7.
Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes. Protein Pept Lett. (2008) 15: 6-12.
Preparation and properties of recombinant rat and human procholecystokinin(57-95). Protein J. (2008) 27: 186-91.
Purification of CK1 by affinity chromatography on immobilised axin. Protein Expr Purif. (2007) 54: 101-9.
A Mu-class glutathione S-transferase from gills of the marine shrimp Litopenaeus vannamei: purification and characterization. J Biochem Mol Toxicol. (2007) 21: 62-7.
The expression and purification of the N-terminal activation domain of the transcription factor c-Myc: a model substrate for exploring ERK2 docking interactions. Protein Expr Purif. (2007) 53: 80-6.
Recombinant omega-conotoxin MVIIA possesses strong analgesic activity. BioDrugs. (2006) 20: 275-81.
Elevated K-ras activity with cholestyramine and lovastatin, but not konjac mannan or niacin in lung--importance of mouse strain. Biochem Pharmacol. (2006) 72: 1749-55.
A radioassay for synaptic core complex assembly: screening of herbal extracts for effectors. Anal Biochem. (2006) 357: 50-7.
A pull-down assay for 5' AMP-activated protein kinase activity using the GST-fused protein. Mol Biotechnol. (2006) 32: 17-21.
Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beads. Anal Biochem. 2005 Dec 1;347(1):67-76.
Expression and purification of the mitochondrial serine protease LACTB as an N-terminal GST fusion protein in Escherichia coli. Protein Expr Purif. (2006) 45: 335-42.
Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5). Protein Expr Purif. (2006) 45: 352-8.
Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2. J Biol Chem. (2005) 280: 35329-36.
Identification and characterization of a selenium-dependent glutathione peroxidase in Setaria cervi. Biochem Biophys Res Commun. (2005) 331: 1069-74.
Recombinant expression of maize nucleotide excision repair protein Rad23 in Escherichia coli. Protein Expr Purif. (2005) 41: 287-97.
An alternative use of basic pGEX vectors for producing both N- and C-terminal fusion proteins for production and affinity purification of antibodies. Protein Expr Purif. (2005) 40: 287-91.
Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Parasite Immunol. (2004) 26: 351-8.
Interaction of the Hsp90 cochaperone cyclophilin 40 with Hsc70. Cell Stress Chaperones. (2004) 9: 167-81.
Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella. Reprod Biol Endocrinol. (2004) 2: 57.
14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain. J Biol Chem. (2004) 279: 26105-14.
Abiotic stress alters transcript profiles and activity of glutathione S-transferase, glutathione peroxidase, and glutathione reductase in Euphorbia esula. Physiol Plant. (2004) 120: 421-433.
Analysis of specific interactions of native protein phosphatase 1 isoforms with targeting subunits. Methods Enzymol. (2003) 366: 156-75.
The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly. J Biol Chem. (2003) 278: 27644-51.
Preparation of biologically active recombinant human progastrin(1-80). J Protein Chem. (2002) 21: 465-71.
Related products:
View-IP™ Protein A Red-Separopore® 4B-CL (SKU # 20181041)
View-IP™ Protein A Green-Separopore® 4B-CL (SKU # 20181108)
View-IP™ Protein A Blue-Separopore® 4B-CL (SKU # 20181105)
View-IP™ Protein G Red-Separopore® 4B-CL (SKU # 20181118)
View-IP™ Streptavidin Red-Separopore® 4B-CL (SKU # 20181103)
View-IP™ Streptavidin Green-Separopore® 4B-CL (SKU # 20181109)
View-IP™ Streptavidin Blue-Separopore® 4B-CL (SKU # 20181106)
View-IP™ His-tag Nickel Red-Separopore® 6B-CL (SKU # 20181117)
View-IP™ Calmodulin Red-Separopore® 4B-CL (SKU # 20181104)
View-IP™ Calmodulin Green-Separopore® 4B-CL (SKU # 20181110)
View-IP™ Calmodulin Blue-Separopore® 4B-CL (SKU # 20181107)
View-IP™ Anti-HA Red-Separopore® 4B-CL (SKU # 20181115)
View-IP™ Anti-DYKDDDDK (FLAG) Red-Separopore® 4B-CL (SKU # 20181114)
View-IP™ Anti-cMyc Red-Separopore® 4B-CL (SKU # 20181113)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™
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