Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Detection Principle:
Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Instrument: Spectrophotometer
Assay Time: 50 min

Tested Sample Types: Animal liver,muscle

Research Area's: Glycolysis & Carbohydrates

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://jels.ub.ac.id/index.php/jels/article/view/298
https://www.researchgate.net/profile/Olatunde_Akanbi/publication/338698785