GnRH-rec (clone A9E4), anti-human

Source:
A BALB/c mouse was immunized with a BSA-conjugated peptide corresponding to amino acids 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) of human GnRH receptor extracellular domain.
Fusion partner: Sp2/0,

Specifications:
A9E4 reacts with GnRH receptors in the anterior pituitary. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular, and intracellular loops, characteristic of G-protein coupled receptors. Some cancers like ovarian and breast cancers sometimes carry GnRH receptors.

Species reactivity:
Positive: human.

Applications:
A9E4 specifically stains human GnRH receptors in the anterior pituitary.
ELISA: +
Frozen sections: +
Paraffin sections: -
Western blot: +

Positive control: Human pituitary gland, ovarian and breast cancers.

References:
- Karande, AA, et al, Molec.Cell.Endocrinol.114: 51-56 (1995).