Goat Anti-Mouse IgG (FC) Separopore® 4B

Goat Anti-Mouse IgG (FC) Separopore® 4B

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SKU
BW-20181091
Catalog Number: BW-20181091
Size(s): 1 mL, 2 mL
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Separopore® 4B Anti-Mouse IgG (FC) Antibody (Polyclonal IgG) (Agarose)

Cat# 20181091

Details
Host: Goat
Isotype: IgG
Class: Polyclonal
Immunogen: Mouse IgG (FC)

Application:
Goat (anti-Mouse) IgG Separopore® 4B is used for immunoprecipitation and Co-immunoprecipitation studies. It is suitable for antibody purification or to remove unwanted cross-reactivity. Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore® 4B. The antibody preparation is specific for the Fc fragment of mouse IgG when tested against purified mouse IgA, IgG, IgM, Fc and kappa and lamda light chains. Affinity purified goat anti-mouse IgG coupled to Separopore® offers the advantage of increased sensitivity for mouse IgG without cross-reactivity to other substances present on the membrane or cell surface.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Matrix: Separopore® 4B (agarose beads, 4%)
Concentration: 25 mg Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Cyanogen bromide
Ligand density: > 5 mg IgG / ml drained gel
Binding capacity: 0.4 mg / ml drained gel
pH stability: 4 – 9 (for use), 2 – 10 (for cleaning)
Flow Specifications: 70 – 140 cm / h
Supplied as 50% slurry in PBS pH 7.4, 0.02% sodium azide
Storage: 4°C

References:
Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol Cell Endocrinol. (1997) 127: 143-54.
Fc-receptor-mediated targeting of antibody-bearing liposomes containing dideoxycytidine triphosphate to human monocyte/macrophages. J Pharm Pharmacol. (1993) 45: 48-53.
Growth of rat-mouse hybridoma cells in immunosuppressed hamsters. An easy and effective method to prepare monoclonal antibodies from heterohybridoma cell lines. J Immunol Methods. (1990) 130: 25-31.
Evidence for interaction of human anti-idiotypic antibodies with CA 125 determination in a patient after radioimmunodetection. Clin Chem. (1990) 36: 164-7.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity. Hybridoma. (1989) 8: 561-7.
Protein A vectorized toxins--II. Preparation and "in vitro" cytotoxic effect of protein A-ricin A chain conjugate on antibody coated human tumour cells. Mol Immunol. (1988) 25: 473-7.
The human B cell-associated antigen CD24 is a single chain sialoglycoprotein. J Immunol. (1986) 136: 3779-84.
Purification of functional, determinant-specific, idiotype-bearing murine T cells. J Exp Med. (1978) 148: 915-24.

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Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B (SKU # 20181081)
Goat Anti-Mouse IgG (Fc)-Red Separopore® 4B (SKU # 20181082)
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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