Goat Anti-Rabbit IgG (H&L) Separopore® 4B

Separopore® 4B Anti-Rabbit IgG (H+L) Antibody (Polyclonal IgG) (Agarose)

Cat# 20181090

Details
Host: Goat
Isotype: IgG
Class: Polyclonal
Immunogen: Rabbit IgG (H+L)

Application:
Binds rabbit IgG to the beads. Used for immunoprecipitation and Co-immunoprecipitation studies (to separate the IgG’s antigen-containing protein from solution). Affinity isolated goat anti-rabbit IgG coupled to cyanogen bromide-activated Separopore® 4B offers the advantage of increased sensitivity for rabbit IgG without cross-reactivity to other proteins. Goat anti-rabbit IgG Separopore® binds with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. The cross reactivity with human immunoglobulins is minimal.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Matrix: Separopore® 4B (agarose beads, 4%)
Concentration: 25 mg Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Cyanogen bromide
Ligand density: ~ 2.4 mg IgG / ml drained gel
Binding capacity: 1 mg rabbit IgG / ml drained gel
pH stability: 4 – 9 (for use), 2 – 10 (for cleaning)
Flow Specifications: 70 – 140 cm / h
Supplied as 50% slurry in PBS pH 7.4, 0.02% sodium azide
Storage: 4°C

References:
Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. J Mol Microbiol Biotechnol. (2003) 5: 161-6.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Detection of glycophorin A-like glycoproteins on the surface of cultured human cells. Int J Biochem. (1989) 21: 635-43.
The isolation of a rat alveolar macrophage lectin. J Biol Chem. (1986) 261: 7440-4.
A micro-scale method for the conjugation of affinity-purified Fab' to beta-D-galactosidase from Escherichia coli. J Biochem. (1985) 98: 1387-94.
Cross-reactivity of contact site A to antibody produced against a stage-specific antigen from a phenol/water extract of Dictyostelium discoideum. J Biochem. (1984) 95: 1355-66.
A lymphocyte cell surface molecule that is antigenically related to actin. J Immunol. (1983) 131: 370-7.

Related products:
Anti-Bovine Albumin (BSA) Separopore® 4B (SKU # 20181059)
Anti-Bovine Albumin (BSA)-Red Separopore® 4B (SKU # 20181089)
Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B (SKU # 20181081)
Goat (anti-Mouse) IgG Separopore® 4B (SKU # 20181091)
Goat Anti-Mouse IgG (Fc)-Red Separopore® 4B (SKU # 20181082)
IgG (Mouse) Separopore® 4B (SKU # 20181078)
IgG (Rabbit)-Separopore® 4B (SKU # 20181015)

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®