Goat HB (Hemoglobin) ELISA Kit
€0.00
In stock
SKU
ELK4257
Catalog Number: ELK4257
Reactivity: Goat
Standard Range: 7.82-500 ng/mL
Sensitivity: 2.7 ng/mL
Information
Request Manual
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Reactivity: Goat
Standard Range: 7.82-500 ng/mL
Sensitivity: 2.7 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin(HB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin(HB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin(HB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Metabolic pathway, Infection immunity, Hematology, Neuro science,
Alternative Names:
Hgb, Haemoglobin, Heterotetramer(αβ)2
Sample Type:
Serum, plasma, erythrocyte lysates.
Assay Type: Sandwich
Standard: 500 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin(HB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin(HB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin(HB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Metabolic pathway, Infection immunity, Hematology, Neuro science,
Alternative Names:
Hgb, Haemoglobin, Heterotetramer(αβ)2
Sample Type:
Serum, plasma, erythrocyte lysates.
Assay Type: Sandwich
Standard: 500 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
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