Green Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181099
Application:
Reactive Green 19-Separopore® 4B-CL is useful for the isolation of nucleotide enzymes, complement factors, human serum albumin. Immobilized green dye binds from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive green dye to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to green dye resin.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Green 19 dye
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Ligand density: 2 – 4 mg Reactive Green 19 / ml drained gel
Binding capacity: 3 – 6 mg human serum albumin / ml drained gel
pH stability: 3 – 13
Storage: 2 – 8 °C
Supplied: as suspension in 0.5M NaCl containing 0.02% Sodium Azide
References:
Separation of G structures formed by a 27-mer guanosine-rich oligodeoxyribonucleotide by dye-ligand affinity chromatography. J Chromatogr A. (1999) 840: 51-8.
4-Hydroxybenzoate 3-geranyltransferase from Lithospermum erythrorhizon: purification of a plant membrane-bound prenyltransferase. Planta. (1998) 205: 407-13.
Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646. J Bacteriol. (1997) 179: 3482-7.
Bovine UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase. I. Purification and subunit structure. J Biol Chem. (1996) 271: 31437-45.
Purification and characterization of threonine dehydrogenase from Clostridium sticklandii. Arch Microbiol. (1995) 163: 286-90.
UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus. J Biol Chem. (1991) 266: 20953-9.
Fetuin and alpha-2HS glycoprotein induce alkaline phosphatase in epiphyseal growth plate chondrocytes. J Cell Physiol. (1991) 149: 222-34.
The affinity purification and characterization of a dehydrogenase from Aspergillus parasiticus involved in aflatoxin B1 biosynthesis. Prep Biochem. (1991) 21: 125-40.
Evaluation of assays for detecting alpha-1-protease inhibitor during purification from rat serum. Anal Biochem. (1991) 193: 260-5.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Yellow-Separopore® 4B-CL (SKU # 20181098)
Brown-Separopore® 4B-CL (SKU # 20181100)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Appearance Form: Liquid
Appearance Color: Dark blue
Brand Name: bioPLUS™, Separopore®
Reactive Green 19-Separopore® 4B-CL is useful for the isolation of nucleotide enzymes, complement factors, human serum albumin. Immobilized green dye binds from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive green dye to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to green dye resin.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Green 19 dye
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Ligand density: 2 – 4 mg Reactive Green 19 / ml drained gel
Binding capacity: 3 – 6 mg human serum albumin / ml drained gel
pH stability: 3 – 13
Storage: 2 – 8 °C
Supplied: as suspension in 0.5M NaCl containing 0.02% Sodium Azide
References:
Separation of G structures formed by a 27-mer guanosine-rich oligodeoxyribonucleotide by dye-ligand affinity chromatography. J Chromatogr A. (1999) 840: 51-8.
4-Hydroxybenzoate 3-geranyltransferase from Lithospermum erythrorhizon: purification of a plant membrane-bound prenyltransferase. Planta. (1998) 205: 407-13.
Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646. J Bacteriol. (1997) 179: 3482-7.
Bovine UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase. I. Purification and subunit structure. J Biol Chem. (1996) 271: 31437-45.
Purification and characterization of threonine dehydrogenase from Clostridium sticklandii. Arch Microbiol. (1995) 163: 286-90.
UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus. J Biol Chem. (1991) 266: 20953-9.
Fetuin and alpha-2HS glycoprotein induce alkaline phosphatase in epiphyseal growth plate chondrocytes. J Cell Physiol. (1991) 149: 222-34.
The affinity purification and characterization of a dehydrogenase from Aspergillus parasiticus involved in aflatoxin B1 biosynthesis. Prep Biochem. (1991) 21: 125-40.
Evaluation of assays for detecting alpha-1-protease inhibitor during purification from rat serum. Anal Biochem. (1991) 193: 260-5.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Yellow-Separopore® 4B-CL (SKU # 20181098)
Brown-Separopore® 4B-CL (SKU # 20181100)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Appearance Form: Liquid
Appearance Color: Dark blue
Brand Name: bioPLUS™, Separopore®
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