GST-Tagged Recombinant Protein Purification OnePass™ Column 1 x 1 ml
€0.00
In stock
SKU
BW-20170004
Application:
OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore® 4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 4% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel. Reduced glutathione is covalently linked to Separopore® 4B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. The OnePass™ prepacked Glutathione- Separopore® spin columns simply purification of GST-tagged proteins.
Technical Specifications:
Ligand: Reduced glutathione (GSH)
Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%)
Average Particle size: 52 - 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Epoxy
Matrix spacer: 12 atoms
Ligand density: 5 – 10 mg GST / ml drained gel
Binding capacity: 8 mg recombinant GST-tagged protein / ml drained gel
pH stability: 3 – 12
Storage: 2 °C – 8 °C
References:
pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production. Protein Expr Purif. (2008) 62: 120-7.
Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli. Protein Sci. (1997) 6: 2180-7.
Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast. Yeast. (1994) 10: 441-9.
Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli. Biochim Biophys Acta. (1995) 1261: 35-43.
GST fusion vector with caspase-6 cleavage site for removal of fusion tag during column purification. Biotechniques. (2005) 38: 360-364.
Bacterial production of recombinant human poly(ADP-ribose) glycohydrolase. Protein Expr Purif. (2011) 75: 230-5.
Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5). Protein Expr Purif. (2006) 45: 352-8.
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. (1988) 67: 31-40.
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol. (2003) 3: 12.
A strategy for high-level expression of soluble and functional human interferon alpha as a GST-fusion protein in E. coli. Protein Eng Des Sel. (2007) 20: 201-9.
Related products
D-Alanine Separopore® 4B-CL (SKU # 20181063)
L-Aspartic Acid Separopore® 4B-CL (SKU # 20181067)
L-Glutamic Acid Separopore® 4B-CL (SKU # 20181068)
Glycine Separopore® 4B-CL (SKU # 20181065)
L-Histidine Separopore® 4B-CL (SKU # 20181069)
L-Lysine Separopore® 4B-CL (SKU # 20181070)
L-Phenylalanine Separopore® 4B-CL (SKU # 20181071)
Poly-L-Lysine Separopore® 4B-CL (SKU # 20181075)
Glutathione Separopore® 4B (SKU # 20181050)
Glutathione Separopore® 4B (Lyophilized) (SKU # 20181077)
Glutathione Separopore® 4B (Microbeads) (SKU # 20181044)
Glutathione Separopore® 4B-CL (Super Flow) (SKU # 20181088)
Glutathione Separopore® 6B-CL (High Resolution) (SKU # 20182013)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Storage: 2-8°C
Brand Name: bioPLUS
OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore® 4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 4% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel. Reduced glutathione is covalently linked to Separopore® 4B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. The OnePass™ prepacked Glutathione- Separopore® spin columns simply purification of GST-tagged proteins.
Technical Specifications:
Ligand: Reduced glutathione (GSH)
Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%)
Average Particle size: 52 - 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Epoxy
Matrix spacer: 12 atoms
Ligand density: 5 – 10 mg GST / ml drained gel
Binding capacity: 8 mg recombinant GST-tagged protein / ml drained gel
pH stability: 3 – 12
Storage: 2 °C – 8 °C
References:
pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production. Protein Expr Purif. (2008) 62: 120-7.
Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli. Protein Sci. (1997) 6: 2180-7.
Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast. Yeast. (1994) 10: 441-9.
Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli. Biochim Biophys Acta. (1995) 1261: 35-43.
GST fusion vector with caspase-6 cleavage site for removal of fusion tag during column purification. Biotechniques. (2005) 38: 360-364.
Bacterial production of recombinant human poly(ADP-ribose) glycohydrolase. Protein Expr Purif. (2011) 75: 230-5.
Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5). Protein Expr Purif. (2006) 45: 352-8.
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. (1988) 67: 31-40.
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol. (2003) 3: 12.
A strategy for high-level expression of soluble and functional human interferon alpha as a GST-fusion protein in E. coli. Protein Eng Des Sel. (2007) 20: 201-9.
Related products
D-Alanine Separopore® 4B-CL (SKU # 20181063)
L-Aspartic Acid Separopore® 4B-CL (SKU # 20181067)
L-Glutamic Acid Separopore® 4B-CL (SKU # 20181068)
Glycine Separopore® 4B-CL (SKU # 20181065)
L-Histidine Separopore® 4B-CL (SKU # 20181069)
L-Lysine Separopore® 4B-CL (SKU # 20181070)
L-Phenylalanine Separopore® 4B-CL (SKU # 20181071)
Poly-L-Lysine Separopore® 4B-CL (SKU # 20181075)
Glutathione Separopore® 4B (SKU # 20181050)
Glutathione Separopore® 4B (Lyophilized) (SKU # 20181077)
Glutathione Separopore® 4B (Microbeads) (SKU # 20181044)
Glutathione Separopore® 4B-CL (Super Flow) (SKU # 20181088)
Glutathione Separopore® 6B-CL (High Resolution) (SKU # 20182013)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Storage: 2-8°C
Brand Name: bioPLUS
| Is Featured? | No |
|---|
Write Your Own Review