GuHCL 6.0M

A widely used protein denaturant
A strong chaotropic agent used at high concentrations for denaturing native proteins and inactivating RNAses.
6M concentration in water.

Specifications: Biotechnology Grade

A260: <0.03%
A230: <0.15
Lead: <0.0005%
Iron: <0.0005%
RNase/DNase Activity: None detected

References:
Mukhopadhyay, A., Inclusion bodies and purification of proteins in biologically active forms. Adv. Biochem. Eng. Biotechnol. 56, 61-109., (1997)
Levine, A.D., et al., High Level expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli. J. Biol. Chem. 270, 7445-7452, (1995)
Rudloph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J. 10, 49-56, (1996)
Naglak, T.J. and Wang, H.Y., Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization. Enzyme Microb. Technol. 12, 603, (1990)
Marston, F.A.O. and Hartley, D.L., Solubilization of protein aggregates. Meth. Enzymol. 182, 264-276, (1990)

Appearance: Liquid
Boiling Point: 132.9 °C at 760 mmHg (Predicted)
Density: 1.34 g/cm3 at 20 °C
HS Code: 29252000
Melting Point: 180-185 °C(lit.)
RTECS Number: MF4300000

Molecular Weight: 95.53
Molecular Formula: CH5N3.HCl
Purity: >99.0%
Storage: 2-8°C
Solubility: Soluble in water (6 M) at 20 °C, ethanol (300 g/l), and alcohol.
Appearance Form: Liquid
EC No.: 200-002-3
Brand Name: bioPLUS