HigherPurity™ Saliva RNA Extraction Kit

HigherPurity™ Saliva RNA Extraction Kit provides an efficient method for RNA Extraction from preserved Saliva samples in our Saliva RNA Sample Collection & Stabilization Kit.

The procedure includes cell lysis, protein removing, RNA precipitation, washing and hydration. Finally, for removal of genomic DNA contamination is used an approach consisting of two sequential filtrations with different MiroSpin columns.

Advantages & Features
- Convenient: ideal for routine work.
- Safe: avoids phenol/chloroform extraction.
- Highly efficient: obtain 10 – 20 µg of genomic DNA from Saliva (average DNA yield will vary depending on the donor).
- High-quality RNA obtained.
- Sample Volume: 600 uL of preserved saliva sample.
- Buffer-based RNA isolation combined with gDNA removal with columns
- Convenient: reduces sample collection distress and blood sample handling.
- Time-saving protocol.

Includes
Includes for 50 rxn:
- 12 mL Precipitation Solution
- 22 mL Binding Buffer
- 10 mL WB1 Buffer
- 50 RNA miniSpin Columns
- 100 Collection tubes
- 50 units gDNA Removal columns
- 30 mg Proteinase K
- 10 mL Water ( Molecular Biology Grade)

Applications
Purification of Total RNA from Saliva of different origins (human or animal).

Shipping & Storage
Shipped in: Ambient Temperature.
Storage: All kit components has different storage conditions and must be stored at Dry place at the Temperature indicated on the kit label. Proteinase K Solution can be stored for several days at 4°C. For longer-term storage, the unused portion of the solution may be stored in aliquots at – 20 °C until needed.