HigherPurity™ Viral RNA Extraction Kit

HigherPurity™ Viral RNA Extraction Kit is designed for the rapid simultaneous purification of viral RNA from cell –free samples such as Serum, Plasma, Urine, Cell free body fluids, Cell Culture supernatants and Rinse liquid from swabs samples.

Viral RNA molecules bind to the silica-based media and impurities such as proteins and nucleases are removed by thorough washing with Wash Buffer. The RNA is then eluted in sterile, RNase free water. The isolated Viral RNA is ready-to-use and should be stored at – 70°C.

The procedure can be used for isolation of viral RNA from a broad range of viruses. However, performance cannot be guaranteed for every virus species and must be validated by the customer. The amount of purified viral RNA depends on the sample type, the virus titer, sample source, transport, storage, and age. The Kit also includes carrier RNA that improves binding and recovery of low-concentrated viral RNA.

Advantages & Features
- Optimized for Coronavirus Applications.
- Efficient.
- Fast results with minimal handling steps.
- Safe: no phenol-chloroform extraction.
- Ready-to-use: viral RNA is suitable for use in RT-PCR and qRT-PCR.

Includes
Includes for 100 rxn:
- 100 MiniSpin Columns
- 200 Collection tubes (2 mL)
- 4 x 100 μL Carrier RNA (5 μg/μL)
- 4 x 10 mL BLY-Buffer
- 33 mL WB1 Buffer
- 20 mL WB2 Buffer
- 10 mL RNase-free Water

Applications
The purified viral RNA is suitable for use in RT-PCR and qRT-PCR and can be used for:
- Viral Load Monitoring.
- Viral Detection.
- Viral Genotyping.

Shipping & Storage
Shipped in: Ambient Temperature. Carrier RNA solution in Gel Pack.
Storage: All components can be stored at Room Temperature, except Carrier RNA solution at –20 °C. If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow cooling to room temperature before use.