Histone H2B (acetyl K20) polyclonal, anti-human, mouse, rat

Background:
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Alternative Name:
Hist1h2ba, H2B 1A, H2B, H2B histone family, H2B2f, H2B2F, H2Ba, H2Bf, HIST2H2BF, histone H2B, histone H2B type 1, Histone H2B type 2-F, MGC131639,

Application Dilution:
WB:1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100

Specificity: Histone H2B (acetyl K20) polyclonal antibody detects endogenous levels of Histone H2B protein only when acetylated at K20.

Immunogen:
recombinant protein

MW: 14 kDa

Swiss-Prot: O60814(Human) P06899(Human) P23527(Human) P33778(Human) P57053(Human) P58876(Human) P62807(Human) Q16778(Human) Q5QNW6(Human) Q8N257(Human) Q93079(Human) Q99877(Human) Q99879(Human) Q99880(Human) Entrez Gene:64647(Mouse) Q00729(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.