Histone H2B Phospho-Regulation Antibody Sampler Kit
€515.00
In stock
SKU
ECM-HK6520
Catalog Number: ECM-HK6520
Size: Kit
Isotype: rabbit polyclonal
Applications: WB, E
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Size: Kit
Isotype: rabbit polyclonal
Applications: WB, E
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Background:
The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of each histone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generate chromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translational modification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibit transcription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine the chromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode of regulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylation include Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increased transcription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reduces transcription of AMPK-dependent genes and lowers cell survival.
Background References
Workman, J.L. & Kingston, R.E. (1998) Annu Rev Biochem. 67:545.
Ajiro, K. et al. (2010) Cell Death Differ. 17(6):984.
Bungard, D. et al. (2010) Science. 329(5996):1201.
Lau, A.T. et al. (2011) J Biol Chem. 286(30):26628.
Buffer/Storage:
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
Product References:
HP4331 Bertoldo, M. et al. (2016) Mol Cell Endocrin. 7207(16):30001 WB: mouse Sertoli cells
HP4331 Lee, J.H. et al. (2015) Nucleic acids res 43(9): 4505. WB: MCF10A
HP4291 Seo, Y. et al. (2013) PLoS ONE 8(8):e75005. WB: MDCK, A549, and HEK293
HP4291 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
HP4331 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of each histone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generate chromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translational modification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibit transcription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine the chromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode of regulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylation include Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increased transcription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reduces transcription of AMPK-dependent genes and lowers cell survival.
Background References
Workman, J.L. & Kingston, R.E. (1998) Annu Rev Biochem. 67:545.
Ajiro, K. et al. (2010) Cell Death Differ. 17(6):984.
Bungard, D. et al. (2010) Science. 329(5996):1201.
Lau, A.T. et al. (2011) J Biol Chem. 286(30):26628.
Buffer/Storage:
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
Product References:
HP4331 Bertoldo, M. et al. (2016) Mol Cell Endocrin. 7207(16):30001 WB: mouse Sertoli cells
HP4331 Lee, J.H. et al. (2015) Nucleic acids res 43(9): 4505. WB: MCF10A
HP4291 Seo, Y. et al. (2013) PLoS ONE 8(8):e75005. WB: MDCK, A549, and HEK293
HP4291 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
HP4331 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
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