Histone H3 (DiMethyl-K36) polyclonal, anti-human, mouse, rat, other (wide range)

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Alternative Name:
H3.4, H3/g, H3FT, H3t

Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100, CHIPseq: 1:20 - 1:50

Specificity: Histone H3 (DiMethyl-K36) polyclonal antibody detects endogenous levels of Histone H3 protein only when Di-methylated at Lys36.

Immunogen:
A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3

MW: 18kDa

Swis Prot.: Q16695

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS, 50% glycerol, pH7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.