Histone H3 (Mono-Methyl R2) polyclonal, anti-human, mouse, rat, other (wide range)
€428.00
In stock
SKU
BS6302
Catalog Number: BS6302
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC, IF, CHIP
Datasheet
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC, IF, CHIP
Datasheet
Background:
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Alternative Name:
H3R2me1
Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200, CHIP: 1:50~1:200
Specificity: Histone H3 (Mono-Methyl R2) polyclonal antibody detects endogenous levels of histone H3 only when Mono-Methyled at Arg2.
Immunogen:
Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, Mono-methylated at R2.
MW: ~ 17 kDa
Swis Prot.: Q16695
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Alternative Name:
H3R2me1
Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200, CHIP: 1:50~1:200
Specificity: Histone H3 (Mono-Methyl R2) polyclonal antibody detects endogenous levels of histone H3 only when Mono-Methyled at Arg2.
Immunogen:
Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, Mono-methylated at R2.
MW: ~ 17 kDa
Swis Prot.: Q16695
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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