Histone H4 (Acetyl-Lys12) polyclonal, anti-human, mouse, rat

Specificity:
Histone H4 (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys12.

Synonyms: H4F2, HIST, HIST1H4A, Histone H4

Immunogen:
The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.

Immunogen Sequence:
10-59Acetyl

Modification Sites: H:K12 M:K12 R:K12

MW (kDa): 11 kDa

NCBI Gene Symbol: HIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4

Database Links:
Gene ID: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8372
Swiss Prot No.: P62805
OMIM No.: 142750/602822/602823/602824/602825/602826/602827/602828/602829/602830/602831/602835
Unigene No.: Hs.143080/Hs.247816/Hs.248172/Hs.248178/Hs.248179/Hs.278483/Hs.46423/Hs.528055/Hs.533295/Hs.55468/Hs.591790/Hs.655235/Hs.662174/Hs.706635/Hs.718935

Application Dilutions: WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.