Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

Detection Principle:
Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Instrument: Biochemistry analyzer, Spectrophotometer
Assay Time: 30 min

Tested Sample Types: Serum

Research Area's: Amino acids & proteins

Shelf Life: 12 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.sciencedirect.com/science/article/pii/S0166432818312646