Horse ANP (Atrial Natriuretic Peptide) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Atrial Natriuretic Peptide(ANP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Atrial Natriuretic Peptide(ANP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Atrial Natriuretic Peptide(ANP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Endocrinology, Cardiovascular biology, Hormone metabolism

Alternative Names:
ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Competitive Inhibition

Standard: 2000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only