Horse-Power™ Green-Taq DNA Polymerase

Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).

GMP-grade reagent also available.

Advantages & Features
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Convenient: available in different concentrations, sizes and solutions.
- Complete solution: includes MgCl2.

Specifications
Assay Conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes
Includes for 500 U:
- 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
- 25 mM MgCl2 (1.5 mL)
- 1.5 mL Buffer (10x)-

Applications
- Routine amplifications.
- Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
- Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
- PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).