Horse-Power™ Taq DNA Polymerase, MasterMix
€0.00
In stock
SKU
P0035
Catalog Number: P0035 (2 x 1.25 mL
Highly Purified (>98%) for Routine Amplifications in Ready-to-use format
Information
Datasheet
Highly Purified (>98%) for Routine Amplifications in Ready-to-use format
Information
Datasheet
Horse-Power™ Taq DNA Polymerase MasterMix (2x) is an optimized ready-to-use MasterMix that contains all PCR reaction components: dNTPs, PCR buffer, Mg2+ and Taq DNA Polymerase. Only primers and template need to be added. The convenient MasterMix (2x) formulation saves time and eliminates the risk of contamination due to a reduced number of pipetting steps required.
Horse-Power™ Taq DNA Polymerase is a versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
GMP-grade reagent also available.
Advantages & Features
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Convenient: available in different concentrations, sizes and solutions.
Specifications
Assay Conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes
2 x 1.25 mL Horse-Power™ Taq DNA polymerase MasterMix (2x) (2 U/μL)
Applications
- Routine amplifications.
- Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
- Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
- PCR fragments amplification for TA or GC cloning.
Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C (NON Frost-Free Freezer).
Horse-Power™ Taq DNA Polymerase is a versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
GMP-grade reagent also available.
Advantages & Features
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Convenient: available in different concentrations, sizes and solutions.
Specifications
Assay Conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes
2 x 1.25 mL Horse-Power™ Taq DNA polymerase MasterMix (2x) (2 U/μL)
Applications
- Routine amplifications.
- Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
- Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
- PCR fragments amplification for TA or GC cloning.
Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C (NON Frost-Free Freezer).
| Is Featured? | No |
|---|
Write Your Own Review