Hot-start KlenTaq
€0.00
In stock
SKU
VN100HS
Inhibition-resistant Taq Pols & Master Mixes
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start KlenTaq DNA Polymerase is a mixture of KlenTaq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures. This prevents primer-dimer formation and spurious amplification, increasing specificity. During normal cycling conditions, the inhibitor releases the enzyme, allowing efficient amplification.
KlenTaq DNA Polymerase is a Taq polymerase variant that lacks 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. Hot-start KlenTaq DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it cannot be used in real-time PCR TaqMan assays, which require 5’-exonuclease activity.
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start KlenTaq DNA Polymerase is a mixture of KlenTaq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures. This prevents primer-dimer formation and spurious amplification, increasing specificity. During normal cycling conditions, the inhibitor releases the enzyme, allowing efficient amplification.
KlenTaq DNA Polymerase is a Taq polymerase variant that lacks 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. Hot-start KlenTaq DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it cannot be used in real-time PCR TaqMan assays, which require 5’-exonuclease activity.
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