Hot-start Omni Klentaq 2
€0.00
In stock
SKU
VN1900HS
Inhibition-resistant Taq Pols & Master Mixes
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start Omni KlenTaq 2 DNA Polymerase is a blend of Omni KlenTaq 2 DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures but releasing the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature, and reducing primer dimer and spurious amplification, thereby increasing specificity.
This enzyme is a new generation mutant of KlenTaq DNA polymerase that can tolerate high levels of PCR inhibitors. It can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but not in TaqMan assays that require 5'-exonuclease activity.
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start Omni KlenTaq 2 DNA Polymerase is a blend of Omni KlenTaq 2 DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures but releasing the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature, and reducing primer dimer and spurious amplification, thereby increasing specificity.
This enzyme is a new generation mutant of KlenTaq DNA polymerase that can tolerate high levels of PCR inhibitors. It can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but not in TaqMan assays that require 5'-exonuclease activity.
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