Hot-start OmniTaq 3
€0.00
In stock
SKU
VN1800HS
Inhibition-resistant Taq Pols & Master Mixes
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start OmniTaq 3 DNA Polymerase is a novel enzyme mixture composed of OmniTaq 3 DNA Polymerase and an aptamer-based inhibitor. This inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures to prevent non-specific amplification such as primer dimer formation, but releases the enzyme during normal cycling conditions to enable efficient amplification. The hot-start feature of this enzyme allows reactions to be set up at room temperature, increasing specificity and sensitivity of PCR.
This enzyme is a new generation mutant of Taq DNA polymerase that is able to tolerate high levels of PCR inhibitors, making it suitable for challenging samples such as blood and soil. It is compatible with real-time PCR using both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assays that require 5'-exonuclease activity.
- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.
Hot-start OmniTaq 3 DNA Polymerase is a novel enzyme mixture composed of OmniTaq 3 DNA Polymerase and an aptamer-based inhibitor. This inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures to prevent non-specific amplification such as primer dimer formation, but releases the enzyme during normal cycling conditions to enable efficient amplification. The hot-start feature of this enzyme allows reactions to be set up at room temperature, increasing specificity and sensitivity of PCR.
This enzyme is a new generation mutant of Taq DNA polymerase that is able to tolerate high levels of PCR inhibitors, making it suitable for challenging samples such as blood and soil. It is compatible with real-time PCR using both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assays that require 5'-exonuclease activity.
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