HotBegan™ Hot Start Taq-DNA Polymerase, MasterMix
€0.00
In stock
SKU
P0030
Catalog Number: P0030 (2 x 1.25 mL (250 rxn)
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg in Ready-to-use Format
Information
Datasheet
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg in Ready-to-use Format
Information
Datasheet
HotBegan™ Hot Start Taq DNA Polymerase MasterMix an optimized ready-to-use solution containing HotBegan™ Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers.
It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Advantages & Features
- Time-saving solution: ready-to-use MasterMix.
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
- Powerful: amplification of targets up to 5 kb.
- Convenient: available in kits and MasterMix solution.
Specifications
Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
- 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
- 2 x 1.25 mL Nuclease-free water.
Applications
- Real time PCR.
- RT-PCR and quantitative RT-PCR.
- Genotyping with Taqman Probes.
- PCR fragments amplification for TA or GC Cloning.
- Amplification from a limited DNA template or low copy number genes.
Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.
It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Advantages & Features
- Time-saving solution: ready-to-use MasterMix.
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
- Powerful: amplification of targets up to 5 kb.
- Convenient: available in kits and MasterMix solution.
Specifications
Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
- 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
- 2 x 1.25 mL Nuclease-free water.
Applications
- Real time PCR.
- RT-PCR and quantitative RT-PCR.
- Genotyping with Taqman Probes.
- PCR fragments amplification for TA or GC Cloning.
- Amplification from a limited DNA template or low copy number genes.
Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.
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