HotBegan™ Hot Start Taq-DNA Polymerase

HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.

Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

Advantages & Features
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
- Powerful: amplification of targets up to 5 kb.
- Convenient: available in kits and MasterMix solution.

Specifications
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Includes
- 100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL)
- 25 mM MgCl2 (1.5 mL)
- 1.5 mL Buffer (10x)

Applications
- qPCR.
- PCR and RT-PCR.
- Genotyping with Taqman Probes.
- PCR fragments amplification for TA or GC cloning.
- Amplification from a limited DNA template or low copy number genes.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.