Human CD73 (Mammalian, C-6His) recombinant protein
€550.00
In stock
SKU
BT-P1005H1
Background Information:
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Target Name: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE, CD-73
Source:
Recombinant Human IL-73 (accession number AAH65937.1) (Trp27-Lys547) with a 6His tag at the C-terminus is expressed in HEK293 cells.
Molecular Weight: :
The 527 amino acid protein has the predicted molecular weight of 58.8kDa. The protein migrates at approximately 62kDa on SDS-PAGE with DTT-reduced condition
Formulation:
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Storage and Handling:
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
Bioactivity:
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is 8000-24000 pmol/min/μg.
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Target Name: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE, CD-73
Source:
Recombinant Human IL-73 (accession number AAH65937.1) (Trp27-Lys547) with a 6His tag at the C-terminus is expressed in HEK293 cells.
Molecular Weight: :
The 527 amino acid protein has the predicted molecular weight of 58.8kDa. The protein migrates at approximately 62kDa on SDS-PAGE with DTT-reduced condition
Formulation:
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Storage and Handling:
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
Bioactivity:
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is 8000-24000 pmol/min/μg.
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