Human DPP8 (Dipeptidyl Peptidase 8) ELISA Kit

Human DPP8 (Dipeptidyl Peptidase 8) ELISA Kit

€0.00
In stock
SKU
ELK4591
Catalog Number: ELK4591
Reactivity: Human
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.117 ng/mL
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Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 8(DPP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 8(DPP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 8(DPP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 8(DPP8) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
DP8, DPRP1, MSTP141, MSTP097, MSTP135, dipeptidylpeptidase 8, Dipeptidyl Peptidase IV-Related Protein-1, Prolyl Dipeptidase DPP VIII

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only
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