Human IgA (clone EBS-huA), anti-human

Source:
A BALB/c mouse was immunized with human salivary proteins.
Fusion partner: SP-2/0.

Specifications:
EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease.

Species reactivity:
Positive: human.

Applications:
EBS-huA detects IgA in frozen and paraffin embedded tissue sections. It can be applied for typing of myeloma proteins in immunoprecipitation and is further suitable for immunoblotting, immunofluorescence and ELISA (quantification of IgA). EBS-huA can also be used as basis for anti-human IgA conjugates.ELISA: +
Flow cytometry: +
Frozen sections: +
Immunoprecipitation: +
Paraffin sections; Citrat
Western blot: +

Positive control: Human mucosal membranes, saliva.

References:
- Biewenga J. et al, Mol. Immunol.23: 761-767 (1986).Biewenga J. et al, Adv. Exp. Med. Biol.216B: 1239-1249 (1987).Mestecky J. et al, J. Immunol. Meth.193: 103-148 (1996).Oortwijn B.D. et al, Mol Immunol.44(5):966-73 (2007).