Human PNX-20 (Phoenixin-20) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNX-20(Phoenixin-20). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PNX-20(Phoenixin-20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PNX-20(Phoenixin-20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PNX-20(Phoenixin-20) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Tumor immunity, Infection immunity, Cardiovascular biology, Rheumatology

Alternative Names:
Small integral membrane protein, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa, MITRAC7, Phoenixin-14, PNX-14, Phoenixin-20, PNX-20, SMIM20, C4orf52

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only