Human respiratory syncytial virus (RSV) PCR Kit CE/IVD
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RealCycler RESV-
Catalog Number: RealCycler RESV-
1 Intended use:
RealCycler RESV is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of RNA from Respiratory Syncytial Virus in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Respiratory Syncytial Virus is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 fluorophore.
3 Technical specifications
Sensitivity: 100 copies/µL.
Specificity: Respiratory Syncytial Virus (fusion protein (F) gene).
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4 Contents
RealCycler RESV includes the AmpliMix, a retrotranscriptase and a RSV RNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
RealCycler RESV is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of RNA from Respiratory Syncytial Virus in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Respiratory Syncytial Virus is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 fluorophore.
3 Technical specifications
Sensitivity: 100 copies/µL.
Specificity: Respiratory Syncytial Virus (fusion protein (F) gene).
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4 Contents
RealCycler RESV includes the AmpliMix, a retrotranscriptase and a RSV RNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
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