Hygromycin B::BSA Conjugate
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In stock
SKU
BW-40810006
Product Description
Hygromycin B is an antibiotic produced by S. hygroscopicus and is an aminoglycoside that kills bacteria, fungi, and higher eukaryotic cells by inhibition of their protein synthesis. It stabilizes the tRNA-ribosomal acceptor site which inhibits translation. It is also active against prokaryotic cells and is used in the lab for selection and maintenance of cells that contain the hygromycin resistance gene.
BSA (bovine serum albumin) is conjugated to hygromycin B (1:126). One or both of the two amine groups in hygromycin B are directly linked to carboxyl group(s) in BSA. Hygromycin B has a molecular weight of 527.5 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS at a pH of 7.4.
Conjugated BSA-hygromycin B is used to recognize hygromycin B-specific antibodies by ELISA and lateral flow based immunoassay. Product can be stored below -20°C for up to 1 year.
Hazmat Shipping : Non-hazardous
Shelf life : 1 year
Storage : -20°C
Brand Name : bioPLUS
Hygromycin B is an antibiotic produced by S. hygroscopicus and is an aminoglycoside that kills bacteria, fungi, and higher eukaryotic cells by inhibition of their protein synthesis. It stabilizes the tRNA-ribosomal acceptor site which inhibits translation. It is also active against prokaryotic cells and is used in the lab for selection and maintenance of cells that contain the hygromycin resistance gene.
BSA (bovine serum albumin) is conjugated to hygromycin B (1:126). One or both of the two amine groups in hygromycin B are directly linked to carboxyl group(s) in BSA. Hygromycin B has a molecular weight of 527.5 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS at a pH of 7.4.
Conjugated BSA-hygromycin B is used to recognize hygromycin B-specific antibodies by ELISA and lateral flow based immunoassay. Product can be stored below -20°C for up to 1 year.
Hazmat Shipping : Non-hazardous
Shelf life : 1 year
Storage : -20°C
Brand Name : bioPLUS
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