IFN-alpha 2 (clone N27), anti-human

Source:
A BALB/c mouse was immunized with E.coli derived recombinant human IFNα2c.
Fusion partner: NS-1.

Specifications:
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFNαfamily has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFNαare lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFNαinclude cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFNαare now known and seem to beexpressed on most cell types. N27 is specific for human IFNα2 and does not cross react with human IFNα1. N27reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.

Species reactivity:
Positive: human.

Applications:
N27 can be used for the detection of human IFNα2 in ELISA and Western blot. It can be paired with IFNα2 mAb N39, to form an EIA to measure IFNα2.
ELISA: +
Frozen sections: +
Pair: +
Western blot: +

Positive control: Human IFNα2, Namalwa and KGI cells.

References:
- Kontsek, P. et al., Mol Immunol.29: 863-870 (1992).
- Kontsek, P. et al., Immunol. Lett.35: 281-284 (1993).